ELISA¼ì²âÊÔ¼ÁºÐÓÃ;¼°Ô­Àí - Î÷ºçÊÐÍø - Powered by Discuz!

Î÷ºçÊÐÍø

±êÌâ: ELISA¼ì²âÊÔ¼ÁºÐÓÃ;¼°Ô­Àí [´òÓ¡±¾Ò³]
×÷Õß: guozhiwei    ʱ¼ä: 2023-2-11 10:07
±êÌâ: ELISA¼ì²âÊÔ¼ÁºÐÓÃ;¼°Ô­Àí

¡¡¡¡ELISA¼ì²âÊÔ¼ÁºÐÓÃ;£º


¡¡¡¡ELISA¼ì²âÊÔ¼ÁºÐÊÇÓÃÓÚÌåÍⶨÐÔ¼ì²âÈËѪÇå»òѪ½¬ÖеĿ¹ÈËÀàÎìÐ͸ÎÑ×£¨HEV£©²¡¶¾ IgM¿¹Ìå¡£BCA Protein Assay Kit (BCAµ°°×Ũ¶È²â¶¨ÊÔ¼ÁºÐ)µÄÏà¹Ø×ÊѶ¿ÉÒÔµ½ÎÒÃÇÍøÕ¾Á˽âһϣ¬´Óרҵ½Ç¶È³ö·¢ÎªÄú½â´ðÏà¹ØÎÊÌ⣬¸øÄúÓÅÖʵķþÎñ£¡

¡¡¡¡ELISA¼ì²âÊÔ¼ÁºÐ¼ì²âÔ­Àí£º ELISA¼ì²âÊÔ¼ÁºÐÓ¦Óö¨ÐÔ¼ÐÐÄÃâÒß¼ì²â¼¼Êõ£¬ÓúϳɵÄHEV¶àëÄ¿¹Ô­°ü±»Î¢¿×°å°åÌõ£¬½«ÑùÆ·»ò±ê׼Ʒ¼ÓÈë¿×Öв¢·õÓý£¬Èç¹ûÆäÖдæÔÚHEV IgM¿¹Ì壬ÕâЩ¿¹Ìå¾Í»áÓëHEV µÄ¶àëÄ¿¹Ô­½áºÏ£¬²¢¹Ì¶¨ÔÚÉÏÃ棬ϴ°å³ýÈ¥ÆäËü·ÇÌØÒìÐÔ¿¹ÌåºÍÑùÆ·ÖеÄÆäËü³É·Ý¡£È»ºó¼ÓÈëÑò¿¹ÈËIgM-HRP£¨À±¸ù¹ýÑõ»¯Îïø£©Ã¸½áºÏÎ¾­µÚ¶þ´Î·õÓýºó£¬Ã¸½áºÏÎï¾Í»áÓë*´Î·õÓý½áºÏÉϵÄHEV IgM¿¹ÌåÏà½áºÏ£¬Ï´°å³ýȥδ½áºÏµÄø½áºÏÎ¼ÓÈëTMB£¨3£¬3¡¯£¬5£¬5¡¯-Ëļ׻ùÁª±½°·£©µ×ÎïÈÜÒº£¬ÔÚµÚÈý´Î·õÓýʱ»á·¢Éúø-µ×Îï·´Ó¦£¬Ö»ÓÐÄÇЩº¬ÓÐHEV IgM¿¹ÌåºÍø½áºÏÎïËùÐγɵĸ´ºÏÎïµÄ¿×²Å»á·¢ÉúÑÕÉ«±ä»¯£¬¼ÓÈëÁòËáÈÜÒºÖÕֹøºÍµ×Îï¼äµÄ·´Ó¦£¬²¢ÔÚ²¨³¤: 450nm´¦²âÁ¿O.D.Öµ,°´ÕÕ±¾HEV IgM¿¹ÌåELISAÊÔ¼ÁºÐµÄ²âÊÔ±ê×¼, O.D.Öµ´óÓÚ»òµÈÓÚCut-OffÖµµÄÑùÆ·±»ÈÏΪÊdzõÊÔÑôÐÔ¡£

¡¡¡¡ÎÒ˾רҵ´úÀíÃÀ¹úR&D¡¢Amresco¡¢Sigma¡¢Pharmacia¡¢AbcamµÈ¸÷*¸ßÖÊÁ¿ÊÔ¼ÁºÐºÍÉúÎï¿ÆÑÐÊÔ¼Á£¬²úÆ·º­¸ÇELISAÊÔ¼ÁºÐ¡¢ÉúÎïÊÔ¼Á¡¢¿¹Ìå¡¢¶ÔÕÕÆ·¡¢ÑªÇ塢ϸ°û¡¢ÅàÑø»ù¡¢ÊµÑéºÄ²ÄµÈ¡£²¢ÇÒÌṩÃâ·Ñ¼¼Êõ·þÎñºÍÖ¸µ¼¡£»¶Ó­¸÷λÀÏʦºÍͬѧǰÀ´×Éѯ
×÷Õß: Íø·²É·ç    ʱ¼ä: 2023-2-11 10:10
ûÊÂÎÒ¾ÍÀ´¿´¿´£¬¹þ¹þ£¡
×÷Õß: ¿ËÁÖ¶Ù    ʱ¼ä: 2023-2-11 10:20
ɳ·¢£¡É³·¢!
×÷Õß: ÌìÑÄ·    ʱ¼ä: 2023-2-11 10:24
Ϊ×Ô¼º¼ÒÏçµÄÉçÇøÍø¹±Ï×µãÁ¦Á¿£¬»Ø¸öÌû×Ó
×÷Õß: ÀËÖÐƯ²´    ʱ¼ä: 2023-2-11 10:28
ûÊÂÎÒ¾ÍÀ´¿´¿´£¬¹þ¹þ£¡
×÷Õß: ÁºÑÅÐÄ    ʱ¼ä: 2023-2-11 10:29
²Å·¢ÏÖ²ýƽҲÓÐÍøÂçƽ̨£¬Í¦ºÃ Ö§³ÖÁË¡£
×÷Õß: ·¹µêÌÓÅܵÄÓã    ʱ¼ä: 2023-2-11 10:30
ºÃºÃ ѧϰÁË È·Êµ²»´í



»¶Ó­¹âÁÙ Î÷ºçÊÐÍø (http://bbs.xihong021.cn/) Powered by Discuz! X3